Journal: European Journal of Medical Research

Article Title: Overexpression of the lncRNA TUG1 regulates high-glucose-induced endocytic clearance dysfunction in HLSECs via the Wnt/β-catenin signaling pathway

doi: 10.1186/s40001-025-03722-w

Figure Lengend Snippet: a, b Relative mRNA expression of PLVAP and CAV-1 in the different groups. c, d Relative protein expression of PLVAP and CAV-1 in each group. e WB bands of target proteins. f Fluorescence microscopy images of Dil-LDL endocytosis in HLSECs under different intervention conditions. g Analysis of extracellular Dil-LDL fluorescence remaining after cellular uptake. Lower values indicate greater Dil-LDL internalization (repeated-measures one-way ANOVA followed by Tukey’s post-hoc test, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, n = 3, Scale bar = 50 μm for all panels)

Article Snippet: GAPDH (bsm-33033 M), antibodies against β-catenin (bsm-55526R), and p-β-catenin (bs-12854R), antibodies against Cyclin D1 (bs-0623R), antibodies against CAV-1 (bsm-60809R), and HRP-conjugated secondary antibodies were purchased from Bioss Biotechnology Co., Ltd. (Beijing, China).

Techniques: Expressing, Fluorescence, Microscopy